Introduction The healing of combat wounds can be complicated by the presence of foreign bodies. The present research aimed to study the regeneration of soft tissue injuries complicated by foreign bodies in the form of fragments of military uniforms.
Methods 60 rats were divided into four groups: intact (Int), control (Con) and two experimental (EG1, EG2). In Con, wounds were sutured without implantation of foreign bodies. A uniform consisting of 100% cotton was used as foreign bodies for EG1 and a uniform consisting of 65% cotton and 35% polyester for EG2. Histological studies were performed according to generally accepted methods. The growth factors wеre determined by ELISA.
Results In EG1, the inflammatory reaction proceeded protractedly, which complicated the development of granulation tissue. In EG2, considering the moderate inflammatory reactions, healing of the wounds became possible. The increase of the growth factors content in Con was maximal at the 15th day: basic fibroblast growth factor (bFGF)—2.2 times and vascular endothelial growth factor (VEGF)—1.6 times (p<0.001). In experimental groups, the bFGF content was maximal at the 60th day and exceeded the normal values by 1.7 times in EG1 and by 2.6 times in EG2 (p<0.001); the level of VEGF was slightly higher than that in healthy animals.
Conclusions The study showed wound healing in all groups. The repair of injuries in rats of EG1 and EG2 was complicated by the textile implant and took longer. The dynamics of the content of growth factors reflected the impairment of wound healing processes. These violations were more pronounced in the EG1 group.
- molecular biology
- wound management
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Contributors SP designed this study and made revisions throughout the article. OL performed the histological examination of the soft tissues and was a major contributor in writing the manuscript. MK contributed to the statistical analysis of the data. RM and VN contributed to the animal experiments. NS performed the determination of the growth factors content. All authors contributed to and approved the final manuscript.
Funding This study was supported by Ministry of Health of Ukraine.
Competing interests None declared.
Patient consent for publication Not required.
Ethics approval Permission of the Ethics and Bioethics Commission of the Kharkiv Medical Academy of Postgraduate Education dated 11/12/2019 was obtained for the study.
Data availability statement Data are available on reasonable request. The datasets during and/or analysed during the current study are available from the corresponding author on reasonable request.
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