Running speed, HR values and energy expenditure during the three loaded runs
| Run distance | 10 km | 15 km | 20 km |
| Average speed (km/hour) | 9.3±0.7*° (9.2 to 9.4) | 8.7±0.7 (8.6 to 8.8) | 8.6±0.6 (8.52 to 8.66) |
| Average speed (min/km) | 6′30″±30″*° (6′30″; 6′25″) | 6′54″±36″ (7′00″; 6’48’’) | 7′00″±24″ (7′05″ to 6′57″) |
| Average HR (b/min) | 175±8.0*° (174 to 176) | 170±8.0 (168 to 170) | 170±9.1 (169 to 172) |
| Average HR (%max) | 92±4.1*° (91.7 to 92.7) | 89±4.2 (88.6 to 89.6) | 89±4.7 (88.7 to 89.9) |
| EE-Eq (kcal/kg/min) | 0.175±0.016*° (0.173 to 0.177) | 0.163±0.016 (0.161 to 0.165) | 0.160±0.013 (0.158 to 0.161) |
| EE-Eq (total kcal) | 1129±59*° (1121 to 1136) | 1703±80° (1693 to 1712) | 2250±115 (2236 to 2264) |
| EE-HRindex (kcal/kg/min) | 0.174±0.011*° (0.172 to 0.175) | 0.163±0.012 (0.162 to 0.165) | 0.162±0.011 (0.160 to 0.163) |
| EE-HRindex (total kcal) | 1125±96*° (1113 to 1137) | 1714±158° (1694 to 1733) | 2289±232 (2260 to 2317) |
Data are presented as mean (X), SD and 95 % CIs (reported in parenthesis). Differences among mean values of speed and HR for the three distances runs were tested by one-way repeated measures analysis of variance (ANOVA). Asterisks and the degree symbol indicate a significant difference from 15 km and 20 km run, respectively. Differences among the mean values of energy expenditure (EE) for the three distances runs, as calculated based on either Epstein equation (EE-Eq) or HR based calculations (EE-HR index) were tested by two-way ANOVA (method of estimate and distance), followed by Holm-Sidak.