Table 2

Summary of E-gene PCR Cq values from SARS-CoV-2 RNA detected samples

MetricCq* value
Overall mean Cq28.93
Overall median Cq30.44
Lowest Cq11.20
Highest Cq40.63†
Lower CI‡ Cq28.51
Upper CI Cq29.77
Lower TI§ Cq15.28
Upper TI Cq37.92
  • *Cq value is defined as the number of PCR cycles required for the fluorescent signal to cross a positive threshold in a 45-cycle assay.

  • †Highest E-gene PCR Cq value from a SARS-CoV-2 RNA detected sample confirmed by the TaqPath assay.

  • ‡CI is calculated using a 95% confidence level for non-parametric data.

  • §Tolerance interval is calculated using a 95% confidence level and 95% coverage level for non-parametric data.

  • Cq, quantitation cycle.