Summary of E-gene PCR Cq values from SARS-CoV-2 RNA detected samples
| Metric | Cq* value |
| Overall mean Cq | 28.93 |
| Overall median Cq | 30.44 |
| Lowest Cq | 11.20 |
| Highest Cq | 40.63† |
| Lower CI‡ Cq | 28.51 |
| Upper CI Cq | 29.77 |
| Lower TI§ Cq | 15.28 |
| Upper TI Cq | 37.92 |
*Cq value is defined as the number of PCR cycles required for the fluorescent signal to cross a positive threshold in a 45-cycle assay.
†Highest E-gene PCR Cq value from a SARS-CoV-2 RNA detected sample confirmed by the TaqPath assay.
‡CI is calculated using a 95% confidence level for non-parametric data.
§Tolerance interval is calculated using a 95% confidence level and 95% coverage level for non-parametric data.
Cq, quantitation cycle.