Details of the 12 samples established as a proficiency testing panel for this study showing sequencing data generated during the deployed phase at sea which was reanalysed using the Epi2Me toolkit and the ARTIC pipeline
Sample ID | Lineage | Clade (variant) | Reads | Coverage | Quality score | Ct value | Original variant assigned | COG variant reported |
Sample 1_Barcode 13 | None | 21J (Delta) | 18 944 | 65% | Poor | 16.72 | Delta | Delta |
Sample 2_ Barcode 14 | None | 21J (Delta) | 12 791 | 72% | Poor | 16.64 | Delta | Delta |
Sample 3_ Barcode 15 | AY.4 | 21J (Delta) | 45 909 | >99% | Good | 20 | Delta | Delta |
Sample 4_ Barcode 16 | AY.126 | 21J (Delta) | 88 382 | >99% | Good | 22.45 | Delta | Delta |
Sample 5_ Barcode 17 | AY.4 | 21J (Delta) | 50 075 | >99% | Good | 25.08 | Delta | Delta |
Sample 6_ Barcode 18 | AY.43 | 21J (Delta) | 49 588 | 98% | Good | 19.63 | Delta | Delta |
Sample 7_ Barcode 19 | AY.46 | 21J (Delta) | 110 459 | >99% | Good | 23.94 | Delta | Delta |
Sample 8_ Barcode 20 | AY.4 | 21J (Delta) | 109 764 | >99% | Good | 24.1 | Delta | Delta |
Sample 9_ Barcode 21 | AY.4 | 21J (Delta) | 41 152 | 97% | Good | 19.08 | Delta | Delta |
Sample 10_ Barcode 22 | BA.1 | 21K (Omicron) | 153 822 | >99% | Good | 26.28 | Omicron | Omicron |
Sample 11_ Barcode 23 | BA.1 | 21K (Omicron) | 74 939 | >99% | Good | 29.96 | Omicron | Omicron |
NTC | N/A | N/A | 2614 | 0% | Good | N/A | N/A | N/A |
The original variant assignment performed using the CLC Genomics Workbench at sea is shown in the final column (COG variant reported). Quality scores were calculated as part of the ARTIC workflow analysis in Epi2Me.
COG, COVID-19 Genomics Consortium; N/A, not applicable; NTC, non-template control.