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Detection by real-time PCR of the Pfcrt T76 mutation, a molecular marker of chloroquine-resistant Plasmodium falciparum strains

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Abstract

An efficient and fast diagnostic assay was developed to detect the point mutation involved in the resistance of Plasmodium falciparum to chloroquine. The test, based upon a real-time PCR principle and performed with the LightCycler system, was carried out on venous blood isolates from 221 cases of falciparum malaria. The assay provided a quick, sensitive and reliable detection of the T76 Pfcrt mutation and the chloroquine resistance. The results of this study suggest that this LightCycler-based technique could be used to optimise the treatment of imported uncomplicated falciparum malaria, and also for the molecular surveillance of drug resistance among imported cases of this disease.

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Acknowledgements

The authors gratefully acknowledge Joëlle Beaudou, Sandra Bernadets, Cécile Escaffre and Cathy Prohèze for technical assistance.

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Authors and Affiliations

  1. Service de Parasitologie, Molecular Diagnosis Unit, Centre Hospitalier Universitaire Rangueil, 31403, Toulouse 4, France

    Aurélia Vessière, Antoine Berry, Richard Fabre, Françoise Benoit-Vical & Jean-François Magnaval

  2. Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077, Toulouse 4, France

    Françoise Benoit-Vical

Authors
  1. Aurélia Vessière
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  2. Antoine Berry
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  3. Richard Fabre
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  4. Françoise Benoit-Vical
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  5. Jean-François Magnaval
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Correspondence to Jean-François Magnaval.

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Vessière, A., Berry, A., Fabre, R. et al. Detection by real-time PCR of the Pfcrt T76 mutation, a molecular marker of chloroquine-resistant Plasmodium falciparum strains. Parasitol Res 93, 5–7 (2004). https://doi.org/10.1007/s00436-004-1096-6

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  • DOI: https://doi.org/10.1007/s00436-004-1096-6

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